Single Cell Isolation Microfluidics

It refers to the process of isolation manipulation and analysis of the cells at single cell resolution in microfluidic chips.

Single cell isolation microfluidics.

131 132 in parallel this technology has been developed commercially by gigagen. In this work a microfluidic method that is capable of isolating single cells in droplets selecting successful edited clones and expansion of these isoclones is introduced. The unique droplet microfluidic technology speeds up the process at the step of isolating activated single antibody secreting cells in picoliter volume chambers from various tissues including blood bone marrow and b cell plasma which allows secreted antibodies to be detected within hours with a variety of bead based and cell based assay formats. Depending on the application single cell analysis chips can include any or all the above mentioned steps.

Single cell analysis has become one of the most important applications of microfluidic technology in recent years. We demonstrate the benefit of this approach to screen antibodies for the specific binding of cancer target cells in a high throughput single cell format which should have immense clinical potential. Using a hybrid microfluidics method droplets in channels can be individually addressed using a co planar electrode system. Dielectrophoretic digital sorting enzymatic digestion facs hydrodynamic traps laser capture microdissection manual picking microfluidics micromanipulation serial dilution and raman tweezers.

Here we present a microfluidic system that can overcome these limitations making use of a dual color signal normalization approach. The throughput of this method was later increased to 10 6 cells by single cell isolation using droplet microfluidics instead of compartmentalization in microwells.