Sirna Library Screening

Sirna library provides a new and efficient way that bring about many original breakthroughs in biology especially for the discovery of new targets combined with.

Sirna library screening.

Silencer sirna is a great choice for economical screening of pre defined gene families and popular pathways like kinases cell surface receptors and dna damage response. The sirna library brings a revolution in the efficiency of functional genomics research. Cancer research 2012 14 r22. Screening often lies at the beginning of most discovery research.

Sirna library minimize time cost of screening using highly functional sirna. Adapted from hu et al. Take advantage of predefined gene families including druggable genes or whole genome. Sirna libraries composed of large numbers of sirnas have been developed and are used to identify sets of genes functionally involved in a particular biological process or disease.

Library screening using genome wide sirnas as well as micrornas allows a researcher to analyze an entire signaling pathway of one or tens of thousands of genes. Genome wide sirna and microrna libraries. Fast and easy online configuration and ordering of plated sirna microrna reagents. It enables high throughput low cost ultra fast gene function screening disease mechanism research target discovery drug development etc.

Rnai screening has allowed researchers to study the functionality of genes in a cell both individually and in. 1 transfection of cells with sirna pools is performed using reverse transfection. The silencer select human apoptosis sirna library provides investigators with a focused sirna screening platform targeting the most up to date apoptosis gene targets thus enabling a detailed analysis of the apoptotic signaling pathway the silencer select human apoptosis sirna library contains three unique non overlapping sirnas per target for 924 genes. To better understand this complexity researchers are moving toward large screening projects to identify candidate genes involved in their biological question of interest.

The network of possible molecular interactions leading to the causal phenotype within a given system pathway or disease is astounding. Sirna library screening protocol. Prepare sirna liposome complexes in the wells of 96 well plates. Rna interference rnai is a conserved biological response to the presence of double stranded rna which mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids and regulates the expression of protein coding genes.

Skip to primary content. A breast cancer cell line was transfected with a sirna library that targeted a variety of specific kinases. Freedom with custom library design.